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In plants, proline synthesis occurs by two enzymatic steps starting from glutamate as a precursor.
The general phenylpropanoid pathway is branched to the biosynthesis of monolignol by two enzymatic steps catalyzed by cinnamoyl-CoA reductase (CCR) and cinnamyl alcohol dehydrogenase (CAD).
Five-hydroxytryptamine, commonly known as serotonin, is produced by two enzymatic steps from the amino acid tryptophan, reactions that occur in the cytoplasm [ 8].
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From quinic acid, it is converted to shikimate by three enzymatic steps using microbial biotransformation [19].
In animals, fungi, and in prokaryotes belonging to the α-proteobacteria (Ferreira et al. 1993; Panek and O'Brian 2002), heme is synthesized by eight enzymatic steps (Fig. 1; Table 1), starting with condensation of glycine and succinyl CoA to form 5-aminolevulinic acid (ALA).
It is synthesized in two enzymatic steps (by the Pfs enzyme and the LuxS enzyme) from S-adenosyl-homocysteine (SAH), resulting in 4,5-dihydroxy 2,3 pentanedione (DPD) which undergoes spontaneous cyclization and is then complexed with borate to form AI-2 (Fig. 1).
These organisms synthesize trehalose in two enzymatic steps catalyzed by maltooligosyl trehalose synthase (TreY), coded by the treY gene, which promotes the transglycosylation of the last glucose moiety at the reduced end of maltodextrins from a α1-α4 to a α1-α1 bond leading to maltooligosyltrehalose, which contains a trehalose moiety at the end of the polymer.
It involves two enzymatic steps catalyzed by trehalose-6-phosphate synthase (TPS) and trehalose-phosphatase (TPP).
GSH synthesis involves two enzymatic steps catalyzed by γ-glutamylcysteine synthetase and glutathione synthetase (44).
In Arabidopsis, the main source of auxin is from tryptophan via two enzymatic steps, catalyzed by tryptophan amino transferases (TAA1, TAR1, and TAR2) [ 34, 35] and the YUCCA (YUC) family monooxygenases [ 36].
This unusual amino acid is not synthesized as a free intermediate but is exclusively formed by a post-translational modification of eIF5A involving two enzymatic steps.
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