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This threshold was determined by two distinct observers and was kept constant for all measurements for the same marker.
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Blind analysis was completed by two observers to ensure minimal observer bias.
The gills were screened by two observers in 2011 and four observers in 2012.
OGAs were performed by two observers, one veterinary neurology resident (observer 1) and one board-certified veterinary neurologist (observer 2).
The observation was made blindly by two independent observers.
The inter-observer variability was assessed by two independent observers.
The number of collaterals was counted by two independent observers.
The daily interactions were recorded by two independent observers.
Analysis will be by two blinded observers.
Quantification was performed by two independent observers.
Immunostaining was scored by two independent observers.
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