Sentence examples for by two changes of from inspiring English sources

The proceedings have advanced in fits and starts, however, and have been marked by two changes of judges and a change in venue.

After fixation, the sections were dehydrated in methyl cellosolve (BDH Chemicals) for 24 hours, followed by two changes of 100% ethanol for 24 hours each at 4°C.

The samples were infiltrated gradually (3:1. 1:1, and 1 3 100% ethanol: Historesin, 24 hours each) with Historesin (Leica Canada, Markham, Ontario), followed by two changes of pure Historesin.

This was followed by two changes of xylene for 30 min each.

Two 10  μm sections were collected and deparaffinised by two changes of xylene and absolute ethanol.

They were dehydrated in steps of 30, 50, 70, 90 and 100% ethanol on ice, followed by two changes of LR-White resin.

Unbound secondary antibodies were removed by three changes of Tris-buffered saline.

Slides were deparaffinized by three changes of xylene (10 minutes each).

Peptides were extracted by three changes of 60%acetonitrile/0.11% TFA, and all extracts were combined and dried in vacuo.

They were first placed in 95% ethanol and then in absolute ethanol for 5 min each, followed by four changes of Histosolve for 5 min each.

The tissue was fixed in 4% formalin for 24 h, then placed onto a Thermoshandon Excelsior processor that passes 20, 90 and four 100% ethanol aliquots through the tissue for an hour each, followed by three changes of xylene for an hour each at a temperature of 40°C, followed by three changes of paraplast for an hour each at 62°C.