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Analysis of cell supernatants reveals that, whereas no EGF is detected, TGF-alpha is released by two cell lines.
In this study, we examined and compared the immune response going on during development of bladder tumours induced by two cell lines: MB49 and its more invasive variant MB49-I.
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The difference between HL60 and HT29 concerning the extent of apoptosis suffered by these two cell lines may be explained by previous studies that have shown HL60 cells to readily apoptose in response to a variety of stimuli, while HT29 cells are generally resistant to apoptosis [11].
The KEGG pathway analyses were performed on the SDE genes in each cell line, which identified the common pathways shared by the two cell lines and found that the most enriched pathways were different between the two cell lines (Sup Figs 2, 3).
Therefore, SM5-1 recognizes an antigen which is differentially expressed by the two cell lines.
The first unexpected finding occurred when the karyotypes of TPC-1 and FB2 were compared, as several unique cytogenetic abnormalities were shared by the two cell lines.
To validate the microarray data, semiquantitative, competitive RT PCR was employed to assess expression of 11 selected genes shared by the two cell lines (Table 2).
Epidermal growth factor receptor signalling is likely to play different roles in the control of PTHrP gene expression and generation of hypercalcaemia by the two cell lines.
To explore the biological significance of the transcriptome response shared by the two cell lines, z-score reports containing the most significant GO terms were generated from the commonly upregulated (Table 1) and downregulated (Table 2) genes.
For this purpose, a new system was developed to produce high titers of the HSV-1 amplicon vector and the results showed that its package efficiency and the titer ratio to HF10 were improved by passage through two cell lines.
By FACS the two cell lines expressed similar amounts of GFP-tagged transgene (Figure 5A).
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