Sentence examples for by two additional washes from inspiring English sources

The column was then washed with four column volumes of lysis buffer followed by two additional washes with lysis buffer containing 10 mM imidazole at pH 6.0.

The post-hybridization washes consisted of two rounds of washes in 50% formamide/2× SSC (v/v), followed by two additional washes in 2× SSC.

The post-hybridisation washes consisted of two rounds of washes in 50%% formamide/2 × SSC (v/v), followed by two additional washes in 2 × SSC.

After hybridization, membranes were washed twice in 2× SSC and 0.1% SDS for 10 min. at room temperature, followed by two additional washes for 20 min at 65°C in 1× SSC and 0.1% SDS solution.

Cells were then washed twice in cold DME + 20% FBS, twice in cold acid buffer (0.2 M acetic acid, 0.5 M NaCl, pH 2.8) to remove extracellularly bound CTxB, followed by two additional washes in cold PBS.

Arrays were subsequently removed from humidified chambers and quickly submerged and washed in 1×SSC and 0.05% SDS for approximately 2 minutes, followed by two additional washes for 2 minutes each in fresh 0.06×SSC.

The sections were rehydrated with absolute ethyl alcohol, followed by two additional washing steps using 1.0 mL of 10% phosphate-buffered saline (PBS; pH 7.2).

This was followed by three additional washes (10 minutes each) with TPBS/0.1% BSA.

The cells were thereafter washed three times with 500 µl of Schneider's Drosophila medium followed by three additional washes with PBS.

After three consecutive 5 min washes in 0.2% Tween 20/PBS, cells were incubated for 1 h with secondary antibody and DAPI followed by three additional washes before mounting [19].

Cysteines were then capped with iodoacetamide, followed by three additional washes with 250 mM ammonium bicarbonate.