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This was followed by two additional rounds of constant volume equilibration simulations to allow the system to reach a stable conformation at 300 K.
FLcDNA clones selected for complete sequence finishing were rearrayed into 384-well plates, followed by two additional rounds of 5'-end and 3'-end sequencing using vector primers.
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This was followed by three additional rounds in February 2009, November 2009 and December 2010 (Table 1).
In this work, we first completed the functional transition by further decreasing the remaining phosphotriesterase activity (∼10-fold) by four additional rounds of directed evolution for maintaining arylesterase but reducing phosphotriesterase activity (Supplementary file 1).
In addition, two additional rounds of cell division were monitored.
Brazil plans to sell six other licenses in two additional rounds the next two months.
Here two additional rounds of subtraction (driver excess: 400 × and 5.000 × in successive rounds) and amplification were performed.
All random walk runs use the confidence threshold heuristic and two additional rounds of insertions.
We used a confidence threshold, with two additional rounds of insertions.
This process was repeated over two additional rounds so that all gilts received all FM routes.
All samples that did not give a reliable result in the first round of genotyping were resubmitted to up to two additional rounds of genotyping.
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