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Based on the high sequence coverage by trypic fragment analysis, two vitellogenins (VTG1, VTG4) could be identified.
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These patterns were analyzed by substructure fragment analysis [27].
The resulting fragments and templates were successfully ligated, generating pAGS7, which was validated by restriction fragment analysis using NsbI.
Two polymorphisms were genotyped by pyrosequencing, and one was by capillary fragment analysis.
These amounts were insufficient to perform further comparative analyses by restriction fragment analysis or Southern blotting.
Repeat lengths were determined by fluorescent DNA fragment analysis using ABI GeneScan software.
DNA fingerprinting by fluorescent amplified fragment analysis was performed as described previously [16], [39].
Microsatellite instability was evaluated by fragment analysis.
Amplification products were visualized by fragment analysis and methylation dosage ratios (Dm) calculated as described [ 12].
The length of exon 19 deletions was assessed by fragment analysis in these samples.
50 μL of this chromatin was used to assess the size of the DNA fragments obtained by High Sensitivity NGS Fragment Analysis Kit (DNF-474) on a Fragment Analyzer™ (Advanced Analytical Technologies, Inc.).
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