In other experiments, tissue samples were pretreated with naloxone (non-selective opioid receptor antagonist), L-NAME (non-selective NO-synthase inhibitor) or GSH (sulfhydryl reagent) for 30 min, followed by treatment with morphine (10 μM).
Dark-adapted NZW rabbits were treated as follows: (1) treatment with morphine (10, 33 or 100 μg, 5 min); (2) treatment with morphine or endomorphin-1 for 5, 15 or 30 min; (3) pretreatment with naloxone (100 μg), l-NAME (125 μg) or reduced glutathione (GSH, 100 μg) for 30 min, followed by treatment with morphine (100 μg, 5 min).
Juul et al. [ 10] reported that Wls was also down-regulated by treatment with morphine in a mouse model of neonatal stress, which suggests that it can promote the inhibition of Wnt secretion.
In conclusion, wls down-regulate expression by treatment with morphine suggesting a decrease downstream Wnt signaling, affecting neuronal development (structure and synaptic organization) and ear development in particular [ 109, 110, 112, 113].
In conclusion, the decrease of dao.1 expression by treatment with morphine in zebrafish embryos contrary to that obtained with rodents opens a door hitherto unknown in the use of zebrafish as a model to study chronic pain and the effect of morphine in relation to the dao.1 gene.
Finally, the down-regulation of dao.1 expression by treatment with morphine in zebrafish embryos contrary to that obtained with rodents opens a door hitherto unknown in the use of zebrafish as a model to study chronic pain and the effect of morphine in relation to this gene.
The treatment with morphine dose dependently reduced the nociception induced by the Ehrlich tumor.
Treatment with morphine has changed that.
A significant place preference was observed following treatment with morphine in wild-type mice, whereas such an effect of morphine was not found in PKCγ knockout mice.
The baby was born drug addicted from maternal oxycodone abuse and developed Neonatal Abstinence Syndrome (withdrawal from drugs) and suffered during three weeks of treatment with morphine.
The treatment with morphine (10 mg/kg, s.c).
