Sentence examples for by treating the samples from inspiring English sources
Finally, the purified PG is obtained by treating the samples in a boiling water bath for 10 min and centrifuging it at 13,000 ×g to remove the contaminating lysozyme.
A secondary fixation step was performed by treating the samples with 2% osmium tetroxide in 0.1 M cacodylate buffer, followed by washing as before and dehydration in ethanol.
Nucleic acids were eliminated by treating the samples with 1% streptomycin sulphate for 15 min, followed by a 10 min centrifugation (11,000×g).
The stability of R-BSX and its mutants against proteolytic degradation was assessed by treating the samples with proteinase K overnight at room temperature, followed by SDS-PAGE.
Any Genomic DNA contamination was removed by treating the samples with DNase I.
Residual RNA was eliminated by treating the samples with RNase H (Ambion).
Excess Fe(III) was removed from the cell pellet by treating the sample with 0.33 M oxalic acid for one hour at 37°C, and then washing it three times by centrifugation with de-ionized water.
By treating the sample with 2-mercapto-ethanol (2-ME) the number of false positive samples decreases [ 19, 56] because IgM is dissociated and IgM cross-reacts with other bacteria more commonly than IgG [ 57].
The cross-linking was reversed by treating the sampled with RNase A and NaCl at a final concentration of 0.3 M overnight at 67° C and subsequent treatment with proteinase K (Fisher Scientific) for 2 h at 45°C.
In essence, by treating the sample at hand as the population, repeated resampling with replacement from this 'population' and calculation of a parameter of interest builds up a picture, the 'empirical distribution' of this parameter, based on so-called ' B bootstrap estimates' of the parameter of interest.
Katahira et al. [6] have studied the chemical stability of Zr-substituted BaCe0.9−x Z xY0.1O3-δ by heat treating the samples at 900 °C (2 h) in 100 % CO2 atmosphere and observed that the material with x = 0.1 decomposed to BaCO3 and CeO2 phases, while the phase of material with x = 0.4 remained same after CO2 treatment, as untreated sample.
