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As has been shown, one of a pair of interacting genes can be expressed permanently but the joint activity can be regulated by transient expression of the other gene [ 24].
Fig. 2 Subcellular localization of OsSUT2 by transient expression of OsSUT2-GFP fusion proteins in barley aleurone layer cells.
TCD10 localization was investigated by transient expression of GFP fusion in tobacco (Nicotiana tabacum) cells, and confocal microscopy as described previously (Jiang et al. 2014a, b).
More advanced knockout constructs additionally carry loxP sites on both sides of the Bsr cassettes for subsequent removal of the selection marker by transient expression of Cre recombinase, thus allowing generation of multiple knockouts using just a single selection marker.
RSPs of the four serotypes were first produced in 293T cells by transient expression of prME genes.
Finally, we demonstrate by transient expression of reporter fusions in protoplasts that localization of LAZ1 is distributed between the cytosol, the plasma membrane and FM4 64 stained vesicles.
We measured the cAMP accumulation induced by transient expression of a Gpr3 plasmid in CHO cells in basal conditions and in presence of 10 µM sphingosine 1-P.
The loss of focal contacts formation in the spreading margins was completely rescued by transient expression of GFP-myosin IIA in COS-7 cells (Fig. 7B).
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Moreover, a plasma membrane localization of OsCPK18 was observed by transient expression assays of green fluorescent protein-tagged OsCPK18 in onion epidermal cells.
Steroid production by the porcine embryo is tightly controlled by the transient expression of aromatase and 17-hydroxylase (P450C17) between days 10 and 13 [ 20, 21, 52].
The reprogramming process is accompanied by the transient expression of DCX, a microtubule-associated protein that is highly expressed in neuroblasts and immature neurons during development and in neurogenic regions of the adult brain.
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