Sentence examples for by transient expression in from inspiring English sources

Different tagged B2 receptors were engineered and their properties were evaluated by transient expression in HEK293S cells.

Fusion proteins of OsGLP8-6 and OsGLP8-12 with C′-terminal green fluorescent proteins (GFP) were visualized by transient expression in the heterologous plant, N. benthamiana (Fig. 6a).

We screened human cementum tumor-derived cDNA libraries by transient expression in COS-7 cells and "panning" with a rabbit polyclonal antibody against a cementoblastoma conditioned media-derived protein (CP).

This chapter covers the methodology we are using for gene discovery in glucosinolate engineering, namely, guilt-by-association-based in silico methods and fast proof-of-function screens by transient expression in Nicotiana benthamiana.

For transient expression of YFP-AtBS14b and YFP-AtBS14a fusion proteins, AtBS14a and AtBS14b ORFs were cloned into pEarly-GW 104 destination plasmid (Bleckmann et al. [2010]), followed by transient expression in N. benthamiana leaves by using the Agrobacterium-mediated transient expression method (Kim et al. [2009]).

This is accomplished by the cloning the novel hemagglutinin gene into a well-characterized vector followed by transient expression in Nicotiana benthamiana plants.

Our result revealed all the five promoter deletion segments were sufficient to drive GUS expression by transient expression both in A. mongolicus and tobacco (Additional file 3).

Because those acylation sites are predicted to be present in grape CDPKs (Table  1), to determine if grape CDPKs localize to membranes, we cloned six VpCDPKs from Chinese wild Vitis pseudoreticulata accession Baihe-35-1 assessedssed the subcellular localizations of the encoded VpCDPKs by transient expression assays in Arabidopsis protoplasts, using translational fusions to GFP.

The repair of 8-OH-Gua was stimulated by addition of purified hOGG1 in vitro and in vivo by transient expression of hOGG1 in HCC1937 cells.

A similar result was obtained by transient expression of Nkx3.1 in ESE3 knock-down LNCaP cells with a significant reduction of their ability to survive in anoikis condition (Fig. 7H).

We confirmed the shRNA resistance by transient expression of FBP1 in FBP-kd cells.