This implies obstruction of the free diffusional movement, either by a network with mesh size of the order of the size of the diffusing proteins, or by transient binding to nuclear structures.
For example, the diffusion of transcription factors is slowed by transient binding to DNA [42], [44].
Our data suggest that HATs may bind transiently to a subset of inactive promoters and acetylated H3K14, which is then removed by the transient binding of HDACs.
The diffusion in the nuclei of living cells is affected by the distribution and the density of the intranuclear obstacles, the transient binding of the proteins to these obstacles, the local viscosity or active transport phenomena.
The apparent discrepancy may be explained by the fact that FRAP experiments mainly detect the bulk, rapid, and potentially transient binding of factors, while ChIP assays only detect productive associations of regulatory genomic regions with specific transcription factors.
In T cells, transient binding of CARMA1 and AIP enhanced formation of the CBM complex.
Only obligate protein complexes were used by Hakes et al. [ 31], and so co-evolution of non-interacting chains cannot occur through the transient binding of other proteins through a single interface.
Transient binding of p53 to repressed promoters might be related to a different mode of transcription activation versus repression.
This likely indicates the transient binding of the ac-tRNA TC to the A site and subsequent rejection.
This conclusion is consistent with evidence of transient binding by Mad2p-GFP and Bub1p-GFP to kinetochores: at peak levels, only 5-10% of spc105-4 and spc105-15 containedtained kinetochore-bound Mad2p-GFP and Bub1p-GFP in contrast to >80% of wild-type cells (Fig. 4B).
