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Cell death in un-transfected time-matched normoxic control cells was <5.0%, and this was not significantly altered by transgene expression (data not shown).
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The transgene expression data for the imprinted L91 lineage were less informative.
In vitro experiments evidenced that these carriers provided high level of transgene expressions (data not shown).
The data show that introduction of transposase by transgene expression or RNA injection results in an even distribution of transposon re-integration events across the zebrafish genome.
Metabolic pathway engineering by transgene expression from the plastid (chloroplast) genome offers significant attractions, including straightforward multigene engineering by pathway expression from operons, high transgene expression levels, and increased transgene containment due to maternal inheritance of plastids in most crops.
Thus, although we lack confirmation by quantitative expression data, it appears that the two GM lines Pm3b#2 and #4, where the transgene showed the strongest phenotypic effects, also had the strongest transgene expression.
These results were further corroborated by low transgene expression and recovery of mutant viral genomes in cardiac and lung tissue following intravenous administration in mice.
The phenotypic and biological defects were rescued by PIG-A transgene expression.
Overall, there is a few available data on transgene expression levels in both stacked and single transgene GM crops in the scientific literature.
The first experiments, carried out in the containment greenhouse at 27-28°C 27-28°Cted to very weak uidA transgene expression, upon GUS histoconductedassay (dato not shown).
typically lost transgene expression by 40 days of selection, probably due to chromatin remodelling and promoter silencing since they remained Zeocin-resistant (data not shown).
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