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By transformation in E.coli TG1 cells a number of colonies much higher than the library complexity was obtained.
We demonstrate by transformation in A. thaliana that a splice site mutation greatly reduces the flowering-repressing activity of this allele.
Purified partial digests were mixed with digested, phosphatased vector DNA in titration experiments and scored by transformation in E coli, solely as a means of determining the number of successful ligations.
A TOPO TA cloning kit was used for ligation, followed by transformation in TOP10 E. coli cells (Invitrogen).
On an underlying basis earnings fell significantly and reflected a year of "transition" to the new superfast broadband technology, to be followed by "transformation" in 2015.
The frequency dependent quantum efficiency, Φ, can be calculated from the photocurrent response to transient excitation by transformation in Laplace space.
This fragment was amplified from pEU720 using primers PTLF2 and PTLR3 ApaI, gel purified, digested with MfeI and ApaI and ligated to MfeI and ApaI digested p567, followed by transformation in E. coli to get the plasmid p1567'.
Full-length human survivin cDNA obtained from Gateway Entry clone (in Gateway vector; Invitrogen, Grand Island, NY, USA) was amplified by transformation in presence of 100 μg ml−1 Kanamycin.
Another case study showed that the 16S rRNA gene could be horizontally transferred by transformation in Helicobacter pylori (Trieber and Taylor 2002).
A segment is aligned with the distance transformation image by translation in a small search window.
apj1 Δ:: URA3 (GSY4341) and gre3 Δ:: URA3 (GSY4324) strains were generated in GSY2719 by transformation with a fusion PCR product (see Table S1 for details).
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