Sentence examples for by transfer to a from inspiring English sources

m7GTP elutions were analyzed on a 10% polyacrylamide gel, followed by transfer to a PVDF membrane.

Cell pellets were lyzed in RIPA buffer and separated on a 10% SDS-polyacrylamide gel followed by transfer to a PVDF membrane (Millipore).

In cortical neurons incubated in EGTA-containing medium, TG treatment followed by transfer to a medium containing CaCl2 in the millimolar range induced Ca2+ influx via SOC channels.

For northern analysis of transfected cell lines, 10 µg of total RNA was resolved on 1.2% denaturing agarose gel electrophoresis followed by transfer to a Hybond N+ membrane (Amersham Biosciences, Piscataway, NJ).

Glycosylation detection and deglycosylation- Twenty micrograms of purified protein were subjected to (i) 10% reducing SDS-PAGE followed by transfer to a PVDF membrane and (ii) glycosylation detection using the ECL glycoprotein detection module according to the manufacturer's protocol.

Immunoprecipitates were collected using Protein G Sepharose (Sigma) and subjected to SDS PAGE, followed by transfer to a nitrocellulose membrane and probed with antibody for the detection of HIF-2α.

Bound proteins were eluted with 40 µl of SDS/sample buffer and separated by 10% SDS-PAGE followed by transfer to an Immobilon membrane (Millipore, MA, USA).

Most centres chose to initiate thrombolysis in the emergency department followed by transfer to an acute stroke or critical care unit for monitoring.

Our query excluded index hospitalizations that were followed by transfer to an acute care setting or resulted in the patient's death during the hospitalization.

He talked about how they were too comfortable in this school, and how he had been "saved" by transferring to a black school after sixth grade.

Here, we've accounted for the Earth's rotation by transferring to a rotating frame of reference - the second term on the right hand side is the Coriolis force, and the third term is the centrifugal force.