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This step was repeated once, followed by transfer through a series (70/30, 30/70, 0/100) to Tris-buffered saline and Tween (TBST; 0.15 M NaCl; 0.2 M Tris buffer, pH 7.5).
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Before embedding in paraffin wax, the tissue samples were dehydrated in an Automatic Tissue Processor (Yorco YSI103LT, Yorco Scientific, India) by transferring through a series of gradually increasing percentages of alcohol.
The embryos were rinsed by transfer through six successive 15-cm tissue culture dishes containing embryo medium.
But he does not endorse cats as a form of pest control because, he explained, the bacteria, viruses, fungi, parasites and nematodes carried by rats may infect humans by secondary transfer through a cat.
Fe(II) can also catalyze the phase transformation from ferrihydrite to goethite and hematite by electron transfer through a dissolution-reprecipitation process [3, 4].
The logic was that many of those passengers, who are paying relatively low fares, could be served by transferring through Atlanta, said Doug Blissit, a Delta vice president.
UB is conjugated to cellular proteins by sequential transfer through an E1-E2-E3 enzymaticascadede.
CO oxidation follows a ping-pong mechanism involving two-electron reduction of the C-cluster followed by electron transfer through an internal electron transfer chain to external electron acceptors.
The two-dimensional model is validated by calculating the heat transfer through a concrete hollow block wall in steady-state and the comparison with experimental results.
Obviously, abovementioned experiment results cannot be accounted for only by the charge transfer through an ideal Schottky barrier.
The AHT used LiBr-H2O to 55%wt for the heat transfer through a heat exchange by falling film.
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CEO of Professional Science Editing for Scientists @ prosciediting.com