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Cellular resistance in vitro and in vivo is correlated with elevated MGMT expression, and can be achieved in previously susceptible cell lines and organisms by transfer and expression of cDNAs or genes encoding MGMT.
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We previously established the ability to recapitulate these natural histone tail activities within non-viral nanocarriers, improving gene transfer and expression by enabling effective navigation to the nucleus via retrograde vesicular trafficking.
The hydrodynamic-based method affords efficient gene transfer and expression by rapid injection of a large volume of DNA solution through the tail vein of an animal.
This study was designed to test the efficiency of therapeutic gene transfer and expression mediated by ultrasound/microbubble strategy in HepG2 cell line.
Kitamura, T. et al. Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics.
RF can enhance gene transfer and gene expression by creating local heat.
Further, peptides isolated by phage display screenings are commonly selected based on receptor binding but not on subsequent internalization, nuclear transfer, and transgene expression.
The first improvement was achieved by transferring the expression cassette from a pBin vector to pTRA, resulting in a substantial increase in recombinant protein yields by transient expression in tobacco.
During exponential growth, 74 probe sets were differentially expressed in REL606 with 17 expression differences appearing by transfer 4 and an additional 57 by transfer 38.
CVE2 showed significantly different levels of expression at a total of 167 probe sets (17 by transfer 4 and an additional 150 by transfer 38; fig. 4).
REL606 showed significantly different levels of expression at a total of 189 probe sets (72 by transfer 4 and an additional 117 by transfer 38) over the course of the experiment (fig. 4).
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