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The annotation of unigenes obtianed by transcriptome sequencing.
Go enrichment of unigenes obtained by transcriptome sequencing.
These efforts were also preceded or accompanied by transcriptome sequencing efforts using different technologies [61 64], in support of gene prediction [65], but also for depicting transcriptional processes and define cell functionality in physiological, pathological, or stress conditions.
Here, the transcriptome of Corynebacterium glutamicum, a non-pathogenic soil bacterium from the actinomycetes branch that is used in industry for the production of amino acids, was analysed by transcriptome sequencing of the 5′-ends of native transcripts.
To validate the differentially expressed genes identified by transcriptome sequencing, we selected genes with fold changes >1.5, p < 0.05, following treatment with both trastuzumab and lapatinib: EGR1, PHLDA1, RGS4, SLC6A6 for qPCR in RNA extracted from two separate batches of iPSC-derived cardiomyocytes (Fig. 4).
The GO term of synapse and synapse part are unique in the transcriptome DETs, with most members first detected by transcriptome sequencing, providing good opportunities to study brain function in the locust phase change.
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We found 74.9% of these ESTs were covered by transcriptome sequences with >80% coverage (Figure S1A).
By transcriptome sequence analysis, we obtained 54,577,780 reads corresponding to about 5 Gb of raw sequence data.
By transcriptome sequence analysis, we obtained 831,632 clean reads, from which 32,759 predicted genes were subjected to BLAST annotation.
Nine genes, identified as differentially expressed between the tame and aggressive fox by transcriptome sequence analysis, were selected for expression evaluation by relative RT-qPCR.
Previously published T. aestivum transcriptome sequences [ 15] were then mapped to all models, which were assigned a confidence value based on the proportion of bases mapped by transcriptome sequences.
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