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Moreover, the availability of EST-linked markers yielded by transcriptome characterisation may provide a suitable tool for the identification of footprints of selective pressures in the released stocks, due to natural or anthropogenic stress.
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In this sense, high throughput technologies supported by systematic cDNA libraries sequencing has been the main approach used for transcriptome characterisation.
How to cite this article: D'Esposito, D. et al. Transcriptome characterisation and simple sequence repeat marker discovery in the seagrass Posidonia oceanica.
Currently, the most common application of NGS in nonmodel species is transcriptome characterisation [ 30- 34].
Therefore, we planned transcriptome characterisation of adult prostate SCs as a strategy to consider new targets for cancer treatment.
The transcriptome characterisation described in the present study will provide the initial information needed for the functional study of persimmons.
Recently, large-scale transcriptome characterisation using the GS FLX platform has been performed for both lentil and pigeonpea [ 8, 37].
Bosco et al. described a genetic induction of CXCR4 in a transcriptome characterisation of monocytes incubated under hypoxia [ 40].
Long reads are optimal for initial genome and transcriptome characterisation because longer pieces are assembled more efficiently than shorter pieces [ 25].
The release of a genome for T. orientalis [ 25] opens new opportunities for tuna comparative genome studies and ongoing transcriptome characterisation projects.
The technology has previously been used to perform de novo bacterial genome sequencing, whole genome shotgun sequencing, metagenomic studies, transcriptome characterisation and small RNA sequence determination [ 26].
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