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Total biofilm biomass (absorbance at OD540) was normalized by total cell mass (absorbance at OD600) to obtain values for normalized biofilm formation and each data point was averaged from 10 replicate wells (five wells per independent culture).
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Then, 2 mL of 0.025 М CdSO4 and 500 μL of 0.5 M Na2S water solutions (Sigma-Aldrich, USA, 98%% purity) were poured into a 100-mL flask with BY-2 cells with total cell mass of 613 mg.
In the REG-80 cultures, only 10 mM of the glycerol substrate was consumed by C. necator H16 by 120 h pi, resulting in a total cell mass accumulation of 2.82 g/L (Fig. 2a).
Similarly, only 7 mM of the glycerol substrate was consumed in the REG-GB cultures by 120 h pi, but the total cell mass accumulation was 3.99 g/L (Fig. 2a).
Genes involved in the planktonic phenotype were identified by screening for Σ1278b mutants with more biofilm-phenotype cells in the total cell mass.
Total cell mass increases exponentially with time.
QX: Biomass productivity at maximum total cell mass produced.
All copy numbers were calculated for the total growing cell mass by multiplying per nucleus copy numbers by total cell numbers.
Importantly, the data have also indicated that the downregulation of MIC-1 in AI PC cells, including PC stem/progenitor cells, may represent a promising therapeutic strategy to improve the anticarcinogenic efficacy of docetaxel, by eradicating the total PC cell mass and thereby preventing disease progression and relapse, and the death of PC patients.
The non-parenchymal areas of the liver are mainly formed by endothelial cells (19%% of the total liver cell mass) (Kmiec 2001).
The age-related growth in total beta cell mass, as shown in Figure 4 (dotted line), can be estimated by the product of the beta cell density and the total volume of the pancreas [10].
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