This observation remained true whether the input animal prion disease brain homogenate was normalized by tissue weight or by PrPres abundance and whether the PMCA substrate was from human brain, PRNP humanized murine brain, or a human-derived and human PrPC overexpressing cell line.
Their bacterial content was estimated by measuring luminescence with an FB12 luminometer (Berthold Detection Systems) normalizing by tissue weight.
For each group, the fluorescence of 12 individual tumor supernatants obtained from three mice per group was normalized by tissue weight prior to analysis.
Median normalization was performed using an in-house-developed R script (Dr. K. Veselkov, Imperial College London), followed by tissue weight normalization.
In humans retinal blood flow volume is likely to be approximately half that of the brain, by tissue weight (i.e. 25:50 ml/100 g/min, inner retinal circulation to total brain) (Sokoloff, 2003; Pournaras et al., 2008).
This was accompanied by greater cecal tissue weight and lower cecal digesta pH (Table 2 ).
Enzyme activity was monitored by recording the changes in absorbance at 412 nm over 2.5 min at 37°C as suggested by Srere (20), normalized to tissue weight, and expressed as micromoles per minute per gram of tissue.
Protective effects of SAME against CCl4 administration on rat thyroid weight, relative tissue weight and % DNA fragmentation by DPA methods are shown in Table 5.
The present study showed that CCl4 increased tissue weight, relative tissue weight, and % DNA fragmentation, which was also revealed by the DNA ladder assay [ 9].
hLPL expression results in decreased plasma TAG levels, elevated FFA uptake by muscle tissue, weight loss and premature death.
