Sentence examples for by three xylene steps from inspiring English sources
Cryosections were mounted on PET-membrane-coated stainless steel slides (Leica Microsystems, Germany) and kept twice for 10 min in 70% ethanol at -20°C followed by three xylene steps for 10 min at room temperature in a fume hood.
The FFPE tissue sections were deparaffinized by three xylene washes, 15 min each.
Tissue was then dehydrated in an ascending series of alcohol (70 100 %), followed by three xylene washes, all for 4 h each.
Tissues were dehydrated in an increasing ethanol series followed by two xylene washes, infiltrated with xylene:paraffin (1∶1, v/v) and two changes of paraffin at 56°C, and embedded for sectioning.
Followed by deparaffinization in three xylene washes and rehydration in graded ethanol, the endogenous peroxidase activity was blocked.
Deparaffinised sections were stained with H&E, followed by three dehydration steps of 60 s each in 70, 95, and 99.5% ethanol, with final dehydration in xylene.
The ethanol was then completely replaced with increasing concentrations of xylene solution followed by a 100% xylene step prior to incubation with paraffin-saturated xylene at room temperature overnight.
The removal of paraffin was carried out by adding 1 ml of xylene for 30 min to the microtube containing the tissue section, followed by two washing steps (30 min each) using 100%and75%5% ethanol.
