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Braasch, I., Volff, J. N. & Schartl, M. The endothelin system: evolution of vertebrate-specific ligand-receptor interactions by three rounds of genome duplication.
Sgc8-FSNPs were collected by three rounds of centrifugation and resuspension in PBS and then stored at 4 °C in MES buffer (0.1 M, pH 5.65) containing 1 % BSA until use.
After she was questioned "like a criminal" over ten hours by three rounds of officers, and "watched as my rape kit was clumsily handled, negligently being tossed around by a policeman," Pepper said an officer insinuated that she was a "party girl," telling her, "You know regrettable sex is not the same as rape".
Cells were lysed routinely by three rounds of sonication, 30s each.
The virus stock was prepared by three rounds of plaque purification in Vero cells.
Recombinant viral isolates were purified by three rounds of plaque purification on Vero cells.
After 48 h cells were collected and lysed by three rounds of freezing and thawing.
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By two rounds of screening, analogue 10 was identified.
Aptamers were developed by nine rounds of SELEX.
The process consists of a written application followed by two rounds of interviews.
We provide evidence that the vertebrate Myb genes arose by two rounds of regional genomic duplication.
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CEO of Professional Science Editing for Scientists @ prosciediting.com