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In most eukaryotic cells, GPCho can also be generated from GPEtn by three methylation steps.
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SAM-dependent methyltransferases play key roles in three methylation steps in caffeine biosynthesis, and catalyze the initial step and the last two steps of purine modification.
Inorganic arsenic is metabolized in two methylation steps catalyzed by AS3MT.
Two methylation steps are required to make the fully methylated oligonucleotide, which can then be restricted by DpnI, resulting in a proportional increase in fluorescence.
However, both theories suggest the two methylation steps are involved in the metabolism of As.
This enzyme is induced by low temperature and catalyzes the three sequential methylation steps to form phosphocholine, a key precursor of phosphatidylcholine and glycinebetaine in plants – metabolites known to be important in conferring tolerance to osmotic stresses such as low temperature, drought and salinity [ 23].
Different N-methyltransferases play important roles in the three N-methylation steps [ 55].
Phospholipid methylation requires three sequential AdoMet-dependent methylation steps to synthesize one molecule of phosphatidylcholine (PC) from phosphatidylethanolamine (PE) de novo.
Purification was performed by three chromatography steps.
However, the structural similarity between N6AMT1 and AS3MT does not include the three cysteine residues (C72, C174, and C224) that bind inorganic AsIII and MMAIII and are necessary for the arsenic methylation steps performed by AS3MT (Marapakala et al. 2012).
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