There's an animation-for-kids show at noon both days, followed by four different programs of experimental animation, music videos and "subversive design works".
Therefore we defined the secretome of each fungal species based on those proteins that are predicted to be secreted by two different programs: SignalP 3.0 [75] and WoLFPSORT [76].
ΔG cost of RNA unfolding calculated as the difference of free energy between optimally folded and completely single stranded local mRNA target was evaluated by two different programs [25]).
RNA secondary structure analysis by two different programs predicted the A allele to alter the stability of a loop in the vicinity of the translation start site.
The SNUGB interface (http://genomebrowser.snu.ac.kr/)[ 15] provides several fields: i) signal peptides predicted by four different programs; ii) effector patterns, such as RXLR and RXLX [EDQ]; iii) nucleotide localization signals predicted by predictNLS; iv) transmembrane helixes predicted by TMHMM 2.0c; and v) hydropathy plots.
Variants were included in this list if they were identified independently by two different programs, SAMtools [ 34] and GATK [ 35] and were characterized by high quality scores as well as presence of reference (B73) and non-reference alleles in the discovery panel.
In silico normalizations were performed using the reads of all the libraries by two different programs in order to produce sequences showing unimodal k-mer coverage distributions with averages around 25: khmer 0.2 with options of "-K 20 –C 20" [ 27] and normalize_by_kmer_coverage.pl implemented in Trinity with options of "--KMER_SIZE 250--max_cov 50" [ 13].
In addition to extensive psychometric evaluations, the subjects in the present study underwent brain anatomical MRI's from which hemispheric grey matter volume (GMV) was measured by two different programs, FreeSurfer (FS) and Voxel-Based Morphology (VBM), implemented in SPM2 [ 54].
The entire sequence of the MTRR gene was scanned by six different programs for splice site prediction (for details see Materials and Methods) and the scores of the splice sites of all the MTRR constitutive exons and the pseudoexon were compared (Table 1 and Supp. Table S1).
The analysis of the 5' UTR for the RNA secondary structure by two different programs showed that the presence of G at -26 stabilized the loop at the vicinity of the translation start site, which could decrease the efficiency of the translation and thereby decrease the expression of BRCA2.
