Sentence examples for by three changes of from inspiring English sources
Unbound secondary antibodies were removed by three changes of Tris-buffered saline.
Slides were deparaffinized by three changes of xylene (10 minutes each).
Peptides were extracted by three changes of 60%acetonitrile/0.11% TFA, and all extracts were combined and dried in vacuo.
The tissue was fixed in 4% formalin for 24 h, then placed onto a Thermoshandon Excelsior processor that passes 20, 90 and four 100% ethanol aliquots through the tissue for an hour each, followed by three changes of xylene for an hour each at a temperature of 40°C, followed by three changes of paraplast for an hour each at 62°C.
After digestion, the peptides were extracted by three changes of 50%acetonitrile/0.1%% formic acid (20 min between changes), dried under vacuum, and resuspended with 10 μL of 0.1% formic acid.
The bones were first dehydrated separately in ethylene glycol (24 h), then washed in 100% ethanol 3 times for 10 min in each, followed by three changes of acetonitrile, a transitional solvent for 15 min in each.
The proceedings have advanced in fits and starts, however, and have been marked by two changes of judges and a change in venue.
After fixation, the sections were dehydrated in methyl cellosolve (BDH Chemicals) for 24 hours, followed by two changes of 100% ethanol for 24 hours each at 4°C.
The samples were infiltrated gradually (3:1. 1:1, and 1 3 100% ethanol: Historesin, 24 hours each) with Historesin (Leica Canada, Markham, Ontario), followed by two changes of pure Historesin.
Two 10 μm sections were collected and deparaffinised by two changes of xylene and absolute ethanol.
