Sentence examples for by three additional passages from inspiring English sources
After initial screening, transformants containing the expected deletion were further purified by three additional passages under selection on solid medium and screened a second time by PCR to check for segregation of the deleted allele.
Transformants containing the expected deletion were further purified by three additional passages on nonselective solid medium and screened by PCR to confirm the absence of the wild-type allele using primers DC409/DC410 to amplify Cbes1854, and DC411/DC412 to amplify a portion of Cbes1855.
Transformants containing the knock-in were further purified by two additional passages under selection on solid medium and screened a second time by PCR.
In the course of experiments, IMR-90 cells were grown for no more than eight additional passages (≤47 total population doublings) and were regularly analysed by standard karyotyping to confirm a normal diploid karyotype.
The original stock of SARS-CoV was subjected to two additional passages in Vero E6 cells.
hMSCs transduced to express either of the four herpesviral immunoevasin genes were kept in culture for five additional passages.
After 5 passages, cells were either frozen for subsequent DNA and RNA analysis, or they were passaged for five additional passages (30 days) without any drug treatment after which they were frozen for future analysis.
Cells were then grown for two additional passages in medium containing HT but lacking Aminopterin, which is the component assimilated by HPRT, to allow TS cells to recover from the selection before they are grown in standard TS medium again.
Frozen cells were kept over a year and displayed the same karyotype upon revival and after five additional passages.
This final movement features bounding, bravura passages for Sebastien Marcovici and brings together dancers from the first movement, which focuses on a lead trio backed up by three additional trios, and the second, which includes a lead couple (Maria Kowroski and Charles Askegard) with seven supporting couples.
This is supported by two additional considerations.
