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This set will produce distinctive hybridization patterns for 634 out of the 889 alleles such that 72.33% of the alleles can not only be detected but also definitively typed; the remaining 255 alleles, while detectable by this probe set, will not hybridize to any unique or unique combination of ultraspecific probe sequences.
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For all "SM" probe sets, the number of probes perfectly matching the genomic sequence within the region defined by the probe set consensus alignment is reported.
The reference set consisted of all genes mapped by the probe set of the microarray used.
Only when the sequence corresponding to a given miRNA binding site was directly recognized by a probe-set or was located upstream to the sequences detected by the probe-set in the gene, did we consider this probe-set to be affected by the activity of this miRNA seed.
We used the following rule for assigning probe-sets to miR-seeds: when the sequence corresponding to a given miR-seed is directly recognized by a probe-set or is located upstream to the sequences detected by the probe-set in the gene and on the same exon, we consider this probe-set to be a reliable indicator of activity for this miR-seed.
The results are validated by the probe sets of S. pombe on the same chips.
No inflammation-associated GO-classification was found uniquely by the probe set-based approach.
Residual heme repression of PrrF expression in the ΔhemO mutant is likely due to overlapping detection of the PrrH RNA by this primer-probe set, as well as effects from contaminating iron in the heme preparation.
This was confirmed by BLASTing these probe set sequences against the reference genome sequences.
SI is calculated by dividing the probe set intensity by the metaprobeset intensity.
PgR: progesterone receptor status represented by PGR Affymetrix probe set 208305_at.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com