Sentence examples for by this normalization factor from inspiring English sources
Subsequently the expression of the gene of interest was divided by this normalization factor.
In order to normalise the control, the signal intensity of each spot of the control was divided by this normalization factor.
(2) We obtained each genome's normalization factor by dividing the above genomic noise factor by the mode of all DGRP genomic noise factors, truncating this normalization factor at 1 (since we are interested only in reducing the influence of nongenuine heterozygosity calls).
In the present work, among the five reference genes tested (Table 1), ubiquitin (UBQ) and cyclophilin (CYP) had the lowest expression stability mean values (M-value = 0.0257), i.e., these genes had more stable expression than other evaluated genes, and the data were normalized by the normalization factor calculated using these most stable reference genes.
The raw Cq value (i.e. non-normalized) for each candidate gene in each sample was divided by the normalization factor (NF). Subsequently, the normalized value for each candidate gene in the target was divided by the normalized value for the corresponding gene in the calibrator to generate relative expression of flavonoid biosynthetic pathway genes in virus-infected leaves.
After re-calculating the normalization factors using geNorm, the highest M value from the three best control genes was reduced to 0.68 and the raw expression values for the genes of interest in each cDNA were divided by the normalization factor for that cDNA to produce the normalized expression data.
Finally the FC of GOI expression was calculated with the ΔCTGOI multiplied by the normalization factor.
For normalization, the highest expression values for each gene were set to 1 and subsequently divided by the normalization factor generated by the GeNorm applet.
The total intensity normalization was carried out by the normalization factor N, defined as the average Cy5 (S-B) divided by the average Cy3 (S-B)).
Normalized read counts were determined by dividing the raw number of reads aligned to each gene by the normalization factor calculated for that gene.
The relative expression for each candidate gene was calculated by dividing the ΔCT of target gene for each sample by the normalization factor of GAPDH and HPRT within the same sample.
