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The estimation of particle sizes by this method requires the solution of an inverse problem using a suitable scattering model that takes into account size, shape and optical properties of the particles.
Hence, vaccination of cattle in the field by this method requires a mixture of parasite strains.
Providing a reliable supply of Cu by this method requires dedicated proton beam and chemistry station that are rarely possible at one of these multipurpose facilities [ 34, 35].
According to Dixon [ 22], optimal threshold calculation by this method requires six responses in the immediate vicinity of the 50% estimated threshold.
Although direct sequencing has generally been used to detect EGFR mutations in previous studies, detection of a mutation by this method requires at least 30% of the mutated DNA in a sample (Bosari et al, 1995; Fan et al, 2001).
Detection of transient perfusion by this method requires that vessels are non-functional for at least 5 min owing to the distribution half-lives of the dyes in the blood.
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However, as indicated by our results, this method requires high RNA-seq read coverage.
Furthermore, as pointed out by the authors, this method requires a relatively higher degree of freedom (the difference between the sample size and the number of phenotypes) in order to be effective.
It is particularly difficult to observe early or fleeting events in cell-cell contact by this approach, since this method requires finding cell pairs which are on the verge of contact, and observing them until they consummate their contact, or do not.
Because available serologic tests are broadly cross-reactive for all New World hantaviruses (18, 19 ), virus identification must be performed by PCR with sequencing; this method requires acute specimens to be collected and transported to the laboratory frozen to preserve RNA for analysis.
Weak indicators can be masked or compensated by other strong indicators; thus this method requires careful implementation.
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