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A large fraction of CNS targets of interest are not suitable for examination by this method, due to the lack of suitable radioligands.
However, not all the relative locations, especially the upstream ones, could be detected by this method due to incomplete annotation of the hookworm genome.
However, it is still difficult to apply genome-wide SNP genotyping analysis to the product obtained by this method, due to the random fragmentation of gDNA and short product length.
The high number of bands generated by automated DNA analyzer indicates the nearly all amplified fragments can be detected by this method, due to better band separation by the DNA sequencing gel.
The data also shows that the NTCD strain cannot be quantified by this method due to absence of toxin production.
Small branches, as defined by a diameter of <15 μm (Glaser et al., 2009), are not resolved by this method due to detection limitations imposed by cast size; however, we define intermediate (20 220 μm) and main (240 520 μm) branches for our analysis.
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Culture-based confirmation, however, is limited by the low yield of this method, due to a high susceptibility to contamination, e.g. after specimen preservation or transportation in sub-optimal conditions, or early antibiotic treatment [9].
The solution of these equations is obtained numerically by the method due to Erdogan, Gupta, and Cook, and the stress intensity factors are displayed graphically.
(Figure S2 shows natural image fragments erroneously labeled as face fragments by the method due to their similarity to actual face fragments).
Chi and CS can be used for PEM formation with the layer-by-layer method, due to the cationic nature of Chi at pH values below 5.0 and the anionic character of CS.
The mutant proteins were not detected by this method, possibly due to co-immunoprecipitation of more abundant proteins of similar molecular weight such as MYH9 (unpublished data).
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