We obtained a predicted conformation for these extensions by performing two rounds of MD simulations.
Poly-A containing mRNA for these samples was isolated by two rounds of binding to Sera-Mag Magnetic Oligo dT) beads, wash and elution in 10 mM Tris buffer pH 7.5, according to manufacturer's instructions (Thermo Scientific, Indianapolis).
The occurrence of conserved paralogous regions on human HOX-bearing chromosomes Hsa 2/7/12/17 has been taken as evidence that these chromosomes are related by two rounds of block, or whole chromosome doubling events [ 8, 11, 16].
These dual-fiber viruses were purified by two rounds of equilibrium centrifugation in CsCl gradients by a standard protocol [43].
AAV vectors were produced by the TIGEM AAV Vector Core by triple transfection of HEK293 cells followed by two rounds of CsCl2 purification (Doria et al, 2013).
AAV2/2 and AAV2/8 vector large preparations were produced by the TIGEM AAV Vector Core by triple transfection of HEK293 cells followed by two rounds of CsCl2 purification.
Now declarer erred by drawing two rounds of trumps.
Vector DNA was isolated by the alkaline lysis method and purified by two rounds of the cesium chloride gradient centrifugation [ 38, 53].
These two pairs of Lx regions were generated by two rounds of polyploidy in soybean.
A series of 52 OTs were examined for allelic deletion by two rounds of microsatellite analysis followed by confirmation with FISH.
