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In the presence of the CGG variant of codon 463, a MspI recognition site is formed, and the two alleles are easily differentiated by their restriction patterns.
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The 27 kb plasmids were digested with EcoRI restriction endonuclease and their restriction patterns compared.
These clones were also grouped into two classes based on their restriction patterns.
Eleven isolates were selected based on their ITS1 restriction patterns.
Based on their distinct restriction patterns, DNA of two isolated plasmids was sequenced.
Strains were characterised by genomic XbaI restriction patterns and resolved through a pulsed field gel electrophoresis and phage typing.
Strains were considered to be of the same cluster if their bands had indistinguishable restriction patterns by both enzymes.
Comparisons were accomplished by aligning the sequence with the optical maps according to their restriction fragment pattern.
Furthermore, the developed method may be used to detect the amplification of various transposable elements ("bursts") by comparison of the restriction patterns of the individual strains and geographical populations of certain species with those of the basic sequenced species strain with partially or completely annotated genome.
The characterization of B. valaisiana was usually proceeded by rRNA gene restriction pattern after digestion by EcoRI and HindIII which contain three and four DNA fragments respectively [ 10], by RFLP of amplicons resulting from intergenic spacer of 23S-5S rRNA which contains four DNA fragments [ 18] or by hybridization on amplicons of 16s RNA gene [ 14].
All 63 recently isolated O139 strains produced identical restriction patterns of their rRNA genes and belonged to ribotype B-II.
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