Sentence examples for by their average Ct from inspiring English sources

The remaining genes were sorted in descending order by their average Ct value obtained from the 15 tumor cell lines.

The genes were sorted in descending order by their average Ct value obtained from the 15 tumor cell lines, and the first 93 genes were selected for further gene expression analysis of patients' samples using the TLDA 96a format (see Additional file 3).

Normalizing the Ct values (per sample) by the Ct of either U6 snRNA (28), the Ct of hsa-miR-24, or their average Ct, shifted at most one sample from each side in the test-set classification predictions.

The CT value of each target was normalized by subtracting the average CT of endogenous control from the CT of each RT-qPCR target.

The expression level of each target gene was normalized against the reference gene YWHAZ (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, QT00105350), calculated as 2−ΔΔCT, where ΔCT was the CT of the target gene after subtracting the CT value of the reference gene and ΔΔCT was the CT value corrected by the average CT of each group.

This observation was independent of the overall expression level as assessed by the average Ct values for each gene.

The ∆Ct is calculated for each individual by the average Ct (threshold cycle) of the tested CNVR – average Ct of a reference gene.

The mRNA levels of GUSB, as determined by the average Ct values for the triplicate reactions, did not differ across LTA4H genotypes in these monocyte samples.

For each reference gene, a correction factor (CF) for each cDNA sample was calculated using the Ct number of this cDNA sample divided by the average Ct number of all cDNA samples included in the same experiment.

In the case of quantitative real-time PCR data, Ct values of the test genes were normalized by the average Ct values of 18S rRNA for each pooled sample.

Expression of transcripts was normalized to the HPRT endogenous gene, and the relative expression was calculated as 2-ΔΔCT, where ΔΔCT is the CT value difference for each patient normalized by the average CT difference of samples from healthy subjects (ΔΔCT method) [ 42].