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The primers were designed by the web based program, Oligo Perfect™ Designer (Invitrogen).
All responses were rendered anonymous by the web based survey platform (Qualtrics Labs, Provo, UT, USA).
RCC1-like repeats for NP_001099274 (R. novegicus Nek8) were analysed by the web based program REP [ 77].
The ratio of synonymous to non-synonymous substitutions was analyzed by the web based algorithm SNAP (http://www.hiv.lanl.gov/content/sequence/SNAP/SNAP.html).hiv.lanl.gov/content/sequence/SNAP/SNAP.html
The sequence logos for R2 and R3 MYB repeats were produced from the multiple alignment files by the web based application WebLogo (http://weblogo.berkeley.edu/logo.cgi) [ 65] using default settings.
(PDF 1497 kb) Additional file 4: Gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) analysis by the web based software ChIP-Enrich of FUS and EWS ChIP-seq peaks [ 51 ]. (DOCX 19 kb) Additional file 5: qPCR validation of enrichment peaks identified by FUS and EWS ChIP-seq.
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Genome annotation, analysis of non coding DNA motifs and functional protein motifs were performed by using the web based gene prediction software GENEMARK http://exon.biology.gatech.edu, the DNASIS MAX 2.00.002.002 software and by sequence comparison.
Of the 512 experts contacted by email, 87 completed the web based survey.
The participants were asked to log in to the web based questionnaire by a personal code enclosed with the information letter.
CAI were calculated for each TvLF gene for all T. vaginalis strains using the web based CAI-Calculator by Puigbo and co-workers [ 55].
The web based application is created by using Apache web server which is connected to the database using MYSQL through an application layer written in Perl-CGI.
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CEO of Professional Science Editing for Scientists @ prosciediting.com