Evidence of this duplication is provided by the very high sequence similarities between these particular domains, as well as from construction of a phylogenetic tree based on their sequences.
However, all of these studies were limited by the very few Hsp90 sequences available at the time.
However, due to the limited information provided by the very short DNA sequences, most current methods are limited in their abilities to detect very short genes.
This was caused not only by the very short-read sequences, but also by intron junctions, which were not included in the short-reads.
Of note, single nucleotide changes may also have been introduced by homologous recombination, given the very high sequence relatedness among most alleles.
However, they are limited by the very small number of sequenced organisms relative to what exists in nature, by the computational complexity of current phylogenetic estimation algorithms, and by the problematic nature of the species concept for bacteria.
This contrast between shallow and deep sequencing results illustrates the effect of sampling on statistical resolution and is mainly influenced by the very small day-260 library sequencing sample.
This is confirmed by the very few number of litchi gene sequences available in public databases, with only 354 nucleotide sequences available in the NCBI GenBank as of 6 Augest 2013 (http://www.ncbi.nlm.nih.gov/GenBank/).nih.gov/GenBank/
The colonies of M. s. schreibersii reveal the moderate differentiation in the control region sequences accompanied by the very strong correlation between the genetic and geographic distances.
Recently, we reported that the very similar sequences differed only by 60 to 80 nt, including an additional 29 nt in the animal virus (6 ).
