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The model fitted properly the data, as was shown by the validation experiments.
Moreover, the adequacy of the models was verified by the validation experiments.
Process authorities can use these models to establish processing guidelines or evaluate current processes for production of shelf-stable pickled carrots or similar foods (as demonstrated by the validation experiments) with pH values from <3.9 to 4.4.
mRNA measurements were excluded if the 18S content fell outside the range in which the PCR was found to be quantitative as defined by the validation experiments.
Pten was one of the genes showing higher AHR binding in naive cells than in BaP- or TCDD-treated cells, a finding that we verified by the validation experiments (p = 0.0025 for BaP and p = 0.0155 for TCDD).
The probes and primers have been validated, and the PCR was shown to be quantitative over a range of 130-fold dilution for ERCC1 and OGG1 and over a 32-fold range for RAI. mRNA measurements were excluded if the 18S content was below the detection level of the individual mRNA defined by the validation experiments.
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The fold increase in HO-1 and NQO1 mRNA due to 5 µM hemin as determined by qRT-PCR in the validation experiments was similar to the expression level identified from the initial microarray analysis (Figure 3A).
However, two miRNAs (miR-496, miR-1538) were found to be upregulated in the old participants by miRNome analysis and in the validation experiments, but when examined among the larger cohort these miRNAs were found to be downregulated in older participants (Fig. 3A, Table S1, S3).
The development of barcode sequencing was led by A.R.G., who also carried out the validation experiments.
Therefore, compared with the validation experiments, the NePhe scoring system is better at keeping hits that are known regulators, while some of these hits are missed by validation experiments.
Therefore, variance analyses were performed by employing the validation experiment (VG) interaction which incorporates: experimenter (PG), array batch (BG), day (AG), and the number of spot pins, coinciding with a change in RNA isolation method (GU).
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Justyna Jupowicz-Kozak
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