Several factors have to be taken into consideration, if implausible results in the transmigration experiments with PCPEC or HIBCPP are observed.
A TEER between 200-300 Ω x cm in the case PCPEC and a TEER between 200-500 Ω x cm in the case of HIBCPP are optimal for performing the transmigration experiments.
As chemoattractant IL-8 (R&D Systems, USA) is used at 10 ng/ml and added to the lower cell culture insert compartment (CSF-side) 30 min before starting the transmigration experiments.
The MLA or CAPE was present throughout the monocytes transmigration experiment until the end.
However, in order to obtain a more direct evidence for the effect of medication on the invasion capacity of tumour cells, transmigration experiments with matrigel invasion chambers were conducted.
In order to strengthen the correlation to the situation in vivo, we conducted additional transmigration experiments with supernatants from human, primary fibroblasts derived from head and neck carcinoma samples.
Paracellular permeability across cell monolayers in the basolateral-to-apical direction is determined during PMN transmigration experiments using Texas Red-labelled dextran (MW 3000; Sigma, Deisenhofen, Germany).
You can watch the The Transmigration of Morton F. online here.
In PMN transmigration experiments, TEER is monitored for 4 h.
In fact, IL-8 induced a strong chemotaxis in our transmigration experiments [ 36].
