Sentence examples for by the transcriptome sequencing from inspiring English sources
The robustness of expression data generated by the transcriptome sequencing was analyzed independently using a qRT-PCR assay.
The RSEM software was run on the data produced by the transcriptome sequencing of M. spicata trichomes by Jin et al. [ 37 ].
Among them, 30 BrkDBD-encoding regions were hit by the transcriptome sequencing data of T. flavidus, whereas none was observed in the transcriptome of T. rubripes.
The 97,565 unigene sequences were downloaded from http://www.genome.clemson.edu/node/273 (presented by the transcriptome sequencing project) (Zhao et al., 2013) and used for subsequent microsatellite mining.
There is a clear agreement between the transcript abundance estimated by the transcriptome sequencing and transcript expression level in the pheromone gland as measured by RT-PCR and qRT-PCR.
For each of the 217 genes called "present" in both the A and B samples on every microarray platform and individual TaqMan assays, the 3.6 million reads generated by the transcriptome sequencing gave at least one hit in both the A and B samples with a maximum of 1944 hits and a median of 55 for the A sample and a maximum of 1226 hits and a median of 46 for the B sample.
By incorporating the transcriptome sequencing results into the comparisons E4/E0 and E24/E0 for all 108 differentially expressed miRNAs under salt stress, we detected 761 and 1,016 miRNA-target pairs, respectively.
The validation of the transcriptome sequencing results by qRT-PCR resulted in an r of 0.68 (Additional file 5), and therewith were in the order of magnitude of results of another RNASeq study in maize [ 31].
Half of the FJ81278 genes received UTR annotations by comparing the transcriptome sequences and the coding regions.
The transcriptome sequences obtained by this study are useful to the understanding of the genetic makeup of the silver carp transcriptome, which until now has been very limited.
This is supported by the recent transcriptome sequencing results of two An.
