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AN participated in the in silico ESTs subtraction analysis of the raw data generated by the transcriptome project.
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The Transcriptome project has been deposited at GenBank PRJNA279853, and the assembled transcriptome at http://evolution.ibmc.up.pt/node/77, or http://dx.doi.org/10.5061/dryad.org/10.5061/dryad
The 97,565 unigene sequences were downloaded from http://www.genome.clemson.edu/node/273 (presented by the transcriptome sequencing project) (Zhao et al., 2013) and used for subsequent microsatellite mining.
Preliminary gene assignments were performed for the tags using parasite full-length genes available in GenBank at the NCBI (nr), followed by TIGR consensus sequences and a clustering of the sequences produced by the S. mansoni transcriptome project [ 5].
Project name: SoyXpress: a database for the soybean transcriptome Project home page:.
The Ipomoea transcriptome is available through the 1kp plant transcriptome project and can be accessed through http://www.onekp.com/.
The 1,000 Plant Transcriptome project [ 33] will also provide transcriptome data for at least 48 conifer species.
To identify 90% of the expressed genes, a transcriptome project should aim at sequencing at least 3 million tags.
Top priority annotation was given to full-length genes, followed by TIGR consensus and our S. mansoni transcriptome project [ 5].
We thus emphasize the need for a specialized HERV Transcriptome Project.
Our results demonstrate that the L. stagnalis CNS transcriptome data complement the transcriptome sequencing project of A. californica [ 38].
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