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The differentially expressed genes have been identified during shedding of immature apple fruits have been identified with a cDNA-AFLP approach [ 20], and the role of NAA and shading in apple fruit abscission have been analyzed by the transcriptome method [ 21].
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Whole transcriptome sequencing was performed as previously described8 using the Transcriptome Capture method.
We identified SNVs (i.e., sites of heterozygosity in our clonal Aiptasia stock) by mapping genomic data to the transcriptome (see Methods).
Metabolic flux analysis furthermore revealed cellular events not predicted by the transcriptome analysis.
Comparison of the transcriptome of zebrafish fully-grown follicles as evaluated by the SAGE method with ovary/egg transcriptomes available for other animal species revealed both similarities and differences.
The same analyses were repeated for gastric cancer transcriptome signature identified by the macrodissection method.
These authors partially explained these major differences by the cultivation method used in different transcriptome experiments.
On the other hand, high-throughput sequencers, typically called next-generation sequencers (NGS), are emerging as a major equipment to acquire information about transcriptomes by the method called mRNA-Seq, because they can be applied not only for well-studied organisms but also for other exotic organisms (Mizrachi et al., 2010; Xu et al., 2011).
Although MPSS and SBS are two different platforms, the transcriptomes generated by the two methods were highly correlated in our study.
The transcriptomes of the Baikal coregonids were compared by the SAGE method [ 22], an experimental technique designed to gain a direct and quantitative measure of gene expression.
In contrast to MPSS, it gives a complex representation of transcriptome as reliable as a LongSAGE library sequenced by the Sanger method.
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