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It is noteworthy that, among the first 12 individual genes with the absolute highest 'A'/'B' ratio between CD34+ and Mk cells, 7 were placed in one significantly over-expressed segment or gene cluster by the transcriptome mapping analysis.
Some of the putative 'non-conserved gene-like sequences' are likely to be pseudogenes or gene fragments, but they also include many expressed genes, as shown by the transcriptome mapping.
Amongst the first 12 genes with the highest 'A'/'B' ratio (PF4V1, PF4, GP1BA, PPBP, RGS18, CMTM5, SLC44A1, VWF, SH3BP5, HSPC159, ITGA2B and HBG1, ranging from 34.22 to 11.92 expression ratio, in this order, between CD34+ and Mk cells), 7 were placed in one significantly over-expressed segment or gene cluster by the transcriptome mapping analysis.
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Then, the transcriptome map was constructed by counting the mapped tags within a sliding window of 100 bp with a 10-bp interval.
As illustrated on chromosome 17 (Fig. 2), the transcriptome map by the real genome data (Fig. 2A) showed various peaks of coexpression on the chromosome.
In contrast, the transcriptome map by the random genome data (Fig. 2B) showed more uniform peaks of coexpression on the chromosome and few peaks were above threshold value.
This distribution is slightly negatively correlated with the GC content (PCC = −0.243), but shows no clear correlation with the transcriptome map by Nagalakshmi et al. (PCC = −0.039; Nagalakshmi et al. 2008).
Several clusters of genes in the transcriptome map showed expression by one virus, but not the other (e.g., MPXV-specific cluster, Figure 6A).
Finally, the transcriptome maps generated using RNA-seq are listed.
In the rpa1a BACs there are indications of mRNA and sRNA variation, as evidenced by the sRNA and transcriptome mapping, that could influence gene expression and function.
Its elucidation will be facilitated by the transcriptome-wide mapping of spliceosome assembly and branch site usage in conjunction with the global analysis of intron retention following loss of particular spliceosome components.
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