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Sequences yielded by the transcriptome assemblies are not necessarily error free but can include incorrect information either caused by the transcription and RNA editing machineries of the plant [ 34, 35], introduced in the sequencing process or resulting from misassemblies.
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We validate our algorithm by simulating the transcriptome assembly of Drosophila using its known genome, and by performing Drosophila transcriptome assembly using publicly available RNA-Seq libraries.
We validate our algorithm by simulating the transcriptome assembly of Drosophila using its known complete genome under the condition that all gene transcripts have high expression levels, and by performing Drosophila transcriptome assembly using publicly available RNA-Seq libraries.
The initial part of the present study was aimed at discovering and characterizing genes from the pigeonpea variety Pusa Ageti by developing and analysing the transcriptome assembly of pigeonpea based on FLX/454 sequencing.
We have demonstrated the reliability of the transcriptome assembly by CEGMA, PCR validation and (q RT-PCR analyses.
This suggests the low DOC in these two genes does not indicate a processing event but is instead a technical inconsistency Potential C→U edit sites were identified in the transcriptome assembly by comparing RNA reads to the published mitogenome sequences (GenBank accessions NC_006581.1 and BA000042).
The completeness of the transcriptome assembly was tested by CEGMA software by comparing known 248 Core Eukaryotic Genes (CEGs) and the transcripts assembled by Trinity.
The transcriptome assembly was conducted by Trinity (r20131110) (Grabherr et al. 2011).
The quality of the transcriptome assembly was tested by comparisons between known genomic sequences of blue crabs and the transcripts assembled by Trinity.
Contigs of the transcriptome assembly were annotated by BLASTX searches with E-value cutoff of 10-5 againsthehe NR protein database to test transcript completeness and diversity.
However, since all loss-of function mutations in the transcriptome assembly are supported by genomic as well as RNA-Seq reads, they are presumably real.
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