Sentence examples for by the threshold cycle from inspiring English sources

The level of the different transcripts was normalized to the level of the GAPDH transcript, and quantified by the threshold cycle Ct method.

Relative gene expression of HRK1 was calculated by the threshold cycle 2−ΔΔCT method [26] from three biological replicates and three technical replicates.

MiRNA expression levels were measured by the threshold cycle (Ct).

Fold changes were determined by the threshold cycle (CT).

The expression levels of miRNAs were measured by the threshold cycle values (Ct).

PCR products were measured by the threshold cycle (CT) values, at which specific fluorescence becomes detectable.

Ptc-1 and Smo mRNA are expressed in human aortic endothelial cells (HAEC) at levels comparable to that of GAPDH, as determined by comparing the threshold cycle numbers obtained by quantitative RT-PCR (Q-RT-PCR) (data not shown).

On the basis of the standard curve of 16S rRNA, the relative mRNA level was determined by calculating the threshold cycle (ΔCt) of each gene by the classic ΔCt method.

Final standard curves were calculated for each pathogen by plotting the threshold cycle value against the bacterial number (log (colony forming units) per milliliter) via linear regression.

Data were normalized by subtracting the threshold cycle (CT) levels between the genes of interest and HPRT [61].

Standard curves were made by plotting the threshold cycle of known CCR5 and HIV-1 copies per reaction.