Injections induced primary hyperalgesia starting after the third injection, accompanied by mild functional gait changes and joint swelling.
Maximal tumour specific antibody (measured by in vivo protection) appeared after the third injection of rabbits for one lymphoma, but after the fifth for another.
In this setting, expression of the hF.IX transgene following the second injection of AAV is expected to be inhibited by the immune response induced by the first injection of AAV.
Wild-type retinas at 4 weeks of age were first injected with ShH10-GFAP-β-catenin, ShH10-GFAP-GFP and ShH10-rhodopsin-tdTomato, followed 2 weeks later by the second injection of ShH10-GFAP-mediated gene transfer of Otx2, Crx and Nrl for rod induction.
Wild-type retinas were injected with ShH10-GFAP-β-catenin (for MG proliferation) and ShH10-rhodopsin-tdTomato (to label MG-derived rods) at 4 weeks of age, followed 2 weeks later by the second injection of ShH10-GFAP-mediated gene transfer of Otx2, Crx and Nrl for rod induction.
Wild-type retinas at 4 weeks of age were first injected with ShH10-GFAP-GFP (label transduced MGs), and ShH10-rhodopsin-tdTomato (label MG-derived rods) in the absence (a f) or presence (g l) of ShH10-GFAP-β-catenin (to stimulate MG proliferation), followed 2 weeks later by the second injection of ShH10-GFAP-mediated gene transfer of Otx2, Crx and Nrl for rod induction.
The increase in intensity sometimes observed during the second administration of contrast agent in the same subject has been related to the saturation of pulmonary macrophages by the first injection, leading to increased signals from the second injection [24].
CFA in the paw or tail produced robust allodynic or hyperalgesic responses, which were completely reversed by the first injection of SNC80 or ARM390 (Figure 2D, Test 1).
The investigational skin test may have induced a transitory immune reaction, which did not influence the skin test result and appeared not to be boosted by the second injection.
At time 0 (8 AM), the rats received the first furosemide injection, followed by the second injection 2 h later (10 AM).
