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The goal of the controlled experiment was to evaluate the SPL use cases' comprehensibility provided by the template structures.
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This is a consequence of the homology modeling method being so highly governed by the template structure.
Finally, comparative models were built for the parts of the sequence aligned by the FR methods to the template structures and the sequence conservation was analyzed in the structural context to detect potential active site residues (see below).
The model is first built by transferring the Cα coordinates of the template structures to the aligned residues in the query sequence.
In a benchmark testing experiment on a set of known proteins, the TM-score of the template structures identified by TM-align (a measure of similarity between model and native with value in 0[ 1]) is shown to be generally lower than that of the TASSER models (only 21% of the TM-align alignments have a higher TM-score, as seen in Figure 4A of [ 6]).
The active site of Glutamate synthase was identified by not only superimposing the template structure (PDB ID: 1E0A) over each other but also by the Parallel-ProBiS algorithm.
The C2A method for instantiating full atomic detail into coarse-grain models is limited by the quality of the template structure and information in the reference full atomic structure.
The position of NOK is decided by superpositioning our model to the template structure 2VC9 (shown in ball and stick).
The Cry1Ab15 resembles the template structure by sharing a common three-domain extending conformation structure responsible for pore-forming and specificity determination.
The high quality of MEDELLER's core models is achieved by reliably selecting parts of the template structure that are similar to the correct target coordinates.
One way by which genes are regulated at the epigenome is through the remodelling of the chromatin histone-DNA complex (the nucleosome), which by post-translational modification changes the template structure of DNA associated histone proteins.
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