Sentence examples for by the temperature shift from inspiring English sources

In the study of the applied process parameters impact, the effect of a higher temperature change rate on austenite kinetics is shown by the temperature shift of austenite and ferrite to austenite start formations.

The replication block of the RM plasmid by the temperature shift decreased its copy number, leading to a decrease in the number of RM molecules, which in turn caused R-mediated breakage of insufficiently methylated genomic sites (Figure 1A, B), leading to the cell death process.

Nitrogen metabolism was also affected by the temperature shift.

Previous studies on the control of gene expression in vector-derived L3 versus 24 hour p.i. L3 highlighted a number of mRNAs which appeared to be regulated largely by the temperature shift associated with infection [ 62].

To investigate whether the DNA-binding activity of NF-YA is required for the p21-mediated PLK1 inhibition, tsp53-H1299 cells were transfected to express wild-type or mutant NF-YA that are impaired in DNA binding (m28 or m29); transfected cells were induced by the temperature shift to activate the p53-p21 ands, and cell lysates were prepared for luciferase assays.

The qRT-PCR results indicated that the CmsHSP1 mRNA levels were elevated already after 5 min of the heat shock (fig. 4 B and C), and the accumulation of the CmsHSP1 protein also began within 10 min (fig. 4 C), confirming the rapid response of the CmsHSP1 gene triggered by the temperature shift (42 50 °C).

Figure 5 shows that the highest cell density could be reached when the temperature-shift was performed to induce expression at an initial cell density OD600 2.0, 2.5, 3.0 or 3.2, but the best enzyme activity could be obtained by performing the temperature shift at about OD600 3.0.

ste11+ mRNA increased 8 hours after Tor2 inactivation in tor2-51, whereas in the tor2-51 pka1Δ ste11+ mRNA levels were already high by 2 hours after the temperature shift.

To ensure that our results in the different cell types were not influenced by reduced cell survival during the temperature shift or transfection procedures we used trypan blue stainings on all cell types to proof cell viability.

Moreover, the elastic modulus of the material can be associated with the temperature by a functional form, known as the temperature shift factor.

Tissue taken from torpid animals was most sensitive to the temperature shift, followed by tissue from aroused and euthermic animals.