Sentence examples for by the target sequence from inspiring English sources
After ligation, the circularized PLP could be primed by the target sequence to initial RCA.
Overall, the signature duration is regulated by the target sequence but not the PEG tag (S1).
In our particular case, one file had sequences composed by the adapter barcode followed by the target sequence (referred to as "adapter read" hereafter), the other file has sequences containing the primer barcode, the IS-Primer, a CA dinucleotide characteristic of the insertion site and the target sequence (called a "primer read" hereafter).
shRNAs against murine β-arrestin1 and CD74 was obtained by cloning the target sequence into pSUPER (Oligoengine Inc., Seattle, WA, USA) RNAi vector.
These results suggest that the differences were determined by either the target sequence or the gRNA.
Since the excision reaction is kinetically favoured over the insertion reaction, gene deletion/inactivation experiments are straightforward to engineer by flanking the target sequence with loxP sites.
SSEARCH was run with default parameters, except for the -z 11 flag, which computes the regression by reshuffling the target sequence library (removing the influence of homologous sequences present within the genome).
shRNA-mediated knockdown of the UHRF1 and FANCD2 genes was achieved by expressing the target sequence 5′-AGATATAACGTTAGGGTTT-3′ and 5′-GAGCAAAGCCACTGAGGTA-3′, respectively, in the pSuper.retro vector (Clontech).
Plasmid pSuper-shESE-1, which was used to generate siRNA against ESE-1, was constructed by inserting the target sequence into pSuper (Oligoengine, USA) in site with Bgl II and Hind III.
The whole SNP data revealed by the target sequencing are available in the dbSNP database [ http://www.ncbi.nlm.nih.gov/projects/SNP/snp_viewTable.cgi handle=CAU_QZHANG].
DNA probe immobilization was carried out electrostatically onto the last PAH layer, followed by hybridization with the target sequence leading to the formation of a partial double stranded (pds) structure onto the films.
