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The histology and grade of the tumor samples were established by the surgical pathologist.
Leftover specimen that was not necessary for diagnosis by the surgical pathologist was used for the imaging process.
However, the assessment of Her2/neu status is hampered by (1) its heterogeneous expression in GC, carrying the risk of a sampling error 3– 14, and (2) by the surgical pathologist's visual perception of what is below and above 10%.
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The patient characteristics including age (median: 50.3 years), menopausal status, clinical stage (TNM classification defined by the International Union against Cancer, UICC, 2003) were assessed by the surgical pathologists.
The diagnoses were confirmed by a surgical pathologist before the tumour samples were harvested for experiments.
Original histopathology data for each case were initially obtained by analyzing hematoxylin and eosin (HE -stained sections from all tumors and tHE -stainedwere further reviewed by a sections pathologist fromthe confirmallon of diagnosis.
The diagnosis is usually confirmed by a surgical pathologist, taking into account the clinical presentation, microscopic findings, and other laboratory tests.
All medical records and cancer sections were examined by a surgical pathologist, and the histological diagnosis and TNM classification were made according to Worldwide Health Organization (WHO) criteria and the classification system of the International Union against Cancer.
Axillary lymph nodes were evaluated immediately by a surgical pathologist at the time of specimen acquisition.
After CARS imaging, the specimens were marked by india blue to indicate the imaged side, sectioned perpendicularly to the microscopic axis, stained with H&E and finally examined by a surgical pathologist to determine the type of tissue as a standard control.
The H&E-stained HNSCC sections were reviewed by a surgical pathologist (KRS) to confirm the diagnosis and determine tumour viability as well as the ratio of tumour to stroma surface area.
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