In addition, by harnessing the structural flexibility and high cross-linking efficiency of BrC6K, we were able to recombinantly staple protein secondary structures and to covalently cross-link native membrane receptors with affibodies in live cells.
However, little importance is usually given to the structural flexibility exhibited by the ∼SbVO4 phase, which has rutile-type structure and can accommodate up to 1/8 of cation vacancies.
As found for sulfoxidation of M213 but to a lesser extent, the replacement of the sulfur atom by a sulfoxide at M206 increases the structural flexibility of the C-terminal part of Helix-2 and the loop connecting Helix-2 and Helix-3 (Figure 2), impacts the general dynamical properties of the protein (Figure 4, Figure 5 and Figure 6) and changes the energetics (Figure 7).
The structural flexibility suggested by models for 5′U AGG/3′G GAU is consistent with NMR spectra as described above.
One study clarified that the structural flexibility caused by Mn2+ is also important for enzyme dynamics, because Mn is required for RNA polymerization [ 4].
The structural flexibility is modelled by the finite element method, and the equations of motion are derived using the Lagrangian formulation.
The structural flexibility of the layered host structure, allows it to adapt to the geometry of the inserted guest species by the free adjustment of the interlayer separation.
In this role, GR acts as an adapter molecule by encoding the ligand's message within the structural flexibility of the ligand binding domain (LBD).
Since UCH DUBs cleave Ub from SUMO2 which contains an unstructured N-terminus, but do not cleave structurally compact Ub dimers, we wanted to determine the contribution of the structural flexibility of the Ub conjugation site to cleavage by UCH DUBs.
Both molecular dynamics and biophysical approaches have shown that the side chain polarity enhancement produced by oxidation impede the native α-fold and provide the structural flexibility required for the acquisition of aggregation properties [31] [34] ,[36]
